Journal: iScience

Article Title: PKR deficiency delays vascular aging via inhibiting GSDMD-mediated endothelial cell hyperactivation

doi: 10.1016/j.isci.2022.105909

Figure Lengend Snippet: Interfering PKR reduces the release of IL-1β and HMGB1 (A) Aortic IL-1β and HMGB1 level in WT and PKR knockout mice of different age (n = 5). (B) IL-1β and HMGB1 secretion in siCon and PKR knockdown (siPKR) HUVECs stimulated with H 2 O 2 (n = 3). ∗∗∗ p< 0.001; ## p< 0.01; ### p< 0.001 (two-way ANOVA test). Data are represented as mean ± SEM.

Article Snippet: Serum or cellular supernatant levels of HMGB1 and IL-1b were measured with ELISA kits (HMGB1 ELISA kit, Shino-Test Corp., Kanagawa, Japan, mouse IL-1b ELISA kit, R&D Systems, USA and Human IL-1β ELISA Kit, Abcam, USA).

Techniques: Knock-Out

Journal: iScience

Article Title: PKR deficiency delays vascular aging via inhibiting GSDMD-mediated endothelial cell hyperactivation

doi: 10.1016/j.isci.2022.105909

Figure Lengend Snippet: Inhibiting endothelial cell hyperactivation prevents the release of IL-1β and HMGB1 (A) Representative immunoblots and densitometric analysis of GSDMD, N-GSDMD, PKR and p -PKR levels in HUVECs with glycine priming and then stimulated with H 2 O 2 (n = 3). (B) IL-1β and HMGB1 secretion in HUVECs with glycine priming and then stimulated with H 2 O 2 (n = 3). (C) Representative immunoblots and densitometric analysis of GSDMD, N-GSDMD, PKR and p -PKR level in HUVECs with disulfiram priming and then stimulated with H 2 O 2 (n = 3). (D) IL-1β and HMGB1 secretion in HUVECs with disulfiram priming and then stimulated with H 2 O 2 (n = 3). ∗∗∗ p< 0.001; ## p< 0.01; ### p< 0.001 (one-way ANOVA test). Data are represented as mean ± SEM.

Article Snippet: Serum or cellular supernatant levels of HMGB1 and IL-1b were measured with ELISA kits (HMGB1 ELISA kit, Shino-Test Corp., Kanagawa, Japan, mouse IL-1b ELISA kit, R&D Systems, USA and Human IL-1β ELISA Kit, Abcam, USA).

Techniques: Western Blot

Journal: iScience

Article Title: PKR deficiency delays vascular aging via inhibiting GSDMD-mediated endothelial cell hyperactivation

doi: 10.1016/j.isci.2022.105909

Figure Lengend Snippet:

Article Snippet: Serum or cellular supernatant levels of HMGB1 and IL-1b were measured with ELISA kits (HMGB1 ELISA kit, Shino-Test Corp., Kanagawa, Japan, mouse IL-1b ELISA kit, R&D Systems, USA and Human IL-1β ELISA Kit, Abcam, USA).

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Staining, Lysis, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Isolation, Transfection, Negative Control, Software, Electron Microscopy

Journal: Journal of Healthcare Engineering

Article Title: Enamel Matrix Derivatives for Periodontal Regeneration: Recent Developments and Future Perspectives

doi: 10.1155/2022/8661690

Figure Lengend Snippet: Recent animal studies and clinical trials on EMD and other therapeutic agents.

Article Snippet: The exact mechanism by which EMD participates in the periodontal regeneration at the cellular and molecular level is still unclear, though Emdogain® (Straumann, Basel, Switzerland), a porcine-derived tooth enamel matrix product, is commercially available with about 15 years of supportive clinical data [ ].

Techniques: Clinical Proteomics, Transplantation Assay